FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length36
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTAGAGGACATGAACAGCAACTTACAAGTCTTA27.6923076923076925No Hit
GTACAATGATGACGCATAGTTCACGATATGTTTCTT13.8461538461538463No Hit
GATATCAATATCCCTACTATCAACAGCGTAACTTGG13.8461538461538463No Hit
GCTGTCGATATTGGGGAACTGTGGTGGTTGGCAAAT13.8461538461538463No Hit
TCCTCAGACTTTCTGATGTCAAATCTTCTAAATTGA13.8461538461538463No Hit
CTTGAAGATGAGACAAGATTGATCGGTTCATGGCAA13.8461538461538463No Hit
GTTCAAGATTTGCCTTATTCTTCTCTATGCTCTTCT13.8461538461538463No Hit
CACCATACCATGCACTTACTTAAATACTATGAACAC13.8461538461538463No Hit
TACTTCTTTAGCGCAAGGGGTAGGATCGATCCACTT13.8461538461538463No Hit
GTGCCAGGGTATCTAGCACGCTCTGCTCAAAAGTCC13.8461538461538463No Hit
TTCCATCACAGCACATTTGATTTCATTTCTGTCGTA13.8461538461538463No Hit
GACAGGTACAATCATATTCTTCATAGTCAGTTTTCT13.8461538461538463No Hit
GTCTTAAAGATTTGAAAAGGTCAACACCGATTTCAC13.8461538461538463No Hit
ATTGGGTAGTGGACAACCAGACAATGACACTGTTGT13.8461538461538463No Hit
CAAGTAGTCCGCCTAGCAGAGCACGCCCCACCAAGC13.8461538461538463No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTA13.8461538461538463No Hit
GTTATATAGTAGAAAGGATGAAGAAAATTTATTCCG13.8461538461538463No Hit
GTGTAGACTTGAGTGACCATTTCATCTTCAATACGA13.8461538461538463No Hit
CTATTGTATCATTTGATACACCAAAAGTGAAATCAT13.8461538461538463No Hit
GAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT13.8461538461538463No Hit
TTCGAATGCTATATCCTCAGTAGCTTAATATTAATC13.8461538461538463No Hit
ACTCTGTTGTGGCACCGCTAACAGTAACGGTGGCCG13.8461538461538463No Hit
CAGCTAATGTTGATGAATCTTCCGCGGAAAACTCTT13.8461538461538463No Hit
GTAGGATTGTATTTTATGTTATGAATATTCGTCCTA13.8461538461538463No Hit
TGATTTGACTATAGATAATTGCTTTTTATCTGCTAT13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers