FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404880
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2300.017.6086355
CGCGGCC550.002103467216.36255813
GCGGGCA650.00639150913.845242513
TATACGG6100.013.0327425
TAGGTAG3250.012.4614955
CGTGCGA850.00261498512.35212810
CTAGGAC1252.5764724E-511.9999593
ACGGTGT6050.011.90029058
GTATTAG6200.011.857511
TACGGTG6100.011.310737
GCAGGCC1602.2653348E-611.2494938
CCCTGCC1755.485854E-711.1421239
GAGCGCA1355.656963E-511.110389
ATAGGGG2301.3151293E-911.0869193
GCGGCCG950.006000914611.05236314
TAATACT12450.010.9638184
GGACCGT1253.4243826E-410.7999636
TCCGAGC1253.4263587E-410.7992913
GTGCGAC3900.010.77008929
TTGGGGG2104.571848E-810.7142494
Produced by FastQC (version 0.11.2)