FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648954
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACC850.00261122412.3545754
GCCTAGG1252.572135E-512.0020381
AGGACAG2701.8189894E-1211.6679885
GTTCTAA6200.011.3728991
CCGTGCA1202.3475388E-411.2493639
ATAGGAC2053.201967E-810.977063
TAGACAG3350.010.7474865
GGCTAGA3101.8189894E-1210.646971
GGACAGT2957.2759576E-1110.1706436
GTATTAG4450.010.1140781
TGGGGGG2854.1472958E-1010.0011335
ACAGTGC4050.09.99943358
TATACTG5400.09.7233235
GCCTAGA1409.703072E-49.6444961
TTAGGGG2251.34541E-69.3345673
CTTACAC4500.09.3345673
CACCGTG1450.00134037639.3096427
GTATAAA7900.09.3053791
TTACACT6000.09.2512234
CTAGGAC1300.0057180899.231993
Produced by FastQC (version 0.11.2)