FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3802653
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG5000.011.1003695
TGTCGGG1253.4267068E-410.79950710
TAAGGCG2957.2759576E-1110.16996429
GTATTAG8050.010.0635051
GTCCTGG2557.188646E-910.0013861
CCGACCT1200.00295797469.9994139
TATACTG13100.09.6186415
GTATAGG5500.09.5467771
TCTTATA12200.09.3444512
GCCCACG3401.10958354E-109.26440518
TAGACCG2957.939889E-99.1528475
ATTATAC12750.09.0590073
TTATACT16300.09.0187054
CGAGCCC3203.3778633E-98.90584415
CTGAGCG1705.777402E-48.823019
TAGACTG5000.08.700295
GTCTAAG6100.08.607751
ACGAGAC3901.8371793E-108.46148722
GTATACG5000.08.4011631
TATACAG14550.08.3507945
Produced by FastQC (version 0.11.2)