FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954740
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCC559.589672E-519.0893619
CGAGACG2700.014.44401423
GAGTGCG750.001019017713.9988679
GCCCACG3250.013.84538718
GGACCGT1106.910146E-613.6373526
CCGAGCC3100.013.54763614
CTCCGAG3250.013.38353112
CCCACGA3300.013.18142519
CCACGAG3200.013.12460820
GTATTAG4450.012.8128671
CGAGCCC3100.012.57994715
ACCGTGC1551.13514034E-712.5796278
TCCGAGC3300.012.27173313
TGGGGGG3150.011.9056245
ACGAGAC3400.011.47024522
CACGAGA3800.011.44702621
CCTGCGA1059.722709E-411.4276468
GACGTAG3550.010.9861526
GAGACGT3650.010.68461324
TCTCCGA4000.010.49914911
Produced by FastQC (version 0.11.2)