FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7390847
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG4700.011.170794
TGGGGGG8400.010.714845
AGGGGGG6600.010.0005175
GTATTAG13250.09.6239851
CTATACT16000.09.5629944
GTATATA33150.09.3678241
TATTATA28350.08.8892882
GTATTAA18850.08.7545391
GTATAAT22650.08.6767071
GTATTAT24900.08.675911
TATACTG19350.08.6050965
GTATAGA17950.08.441291
TCTATAC16200.08.4263043
TCTTATA22750.08.2421282
TATACCG7300.08.2196045
TTATACT27650.08.1921054
CCGTGCA3501.6802005E-88.1423419
GTATTAC15550.08.1040261
TTAGGGG5950.08.0675893
TAAGACT16700.07.90464
Produced by FastQC (version 0.11.2)