FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134134
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG650.006390147413.8458759
CGAGCCC1753.081368E-912.85647315
CCGAGGC1857.030394E-912.16288828
TCCGAGC2052.568413E-911.70708113
GCCGTGC1059.720615E-411.4283418
CTATGCG1653.2772186E-610.9088719
GACCGTG1408.1878694E-510.7137277
CCGAGCC2002.691795E-710.49978814
CCGTGCA1901.5807327E-610.262959
GACCGAG2209.037467E-810.22788126
GCCCACG2352.1700544E-810.212234518
CTCCGAG2803.0013325E-1010.17836612
GTATTAG7050.09.362411
GCAGCGT2752.4698238E-89.2734281
TGGGGGG3759.094947E-129.2001085
GTATTAT13050.09.1960961
CTAAGGC4250.09.17657853
TATACTG11000.09.1364715
CGAGGCT2352.4305282E-68.93670329
GTATATA21500.08.9309061
Produced by FastQC (version 0.11.2)