FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152303
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1301.12504495E-815.0016285
GTGGGCA1006.4525194E-411.9992199
GTATAGG2154.274625E-1011.8622671
GGACAGC900.004003847511.6679326
TCTTATA2605.456968E-1211.5397142
TGTACGT1303.8460654E-511.53771110
GTCTGTC1059.716118E-411.4278277
GTATTAA2900.011.3810391
GGTCGTG950.005999526511.0519127
ACCACGC950.005999526511.0519128
ACGGAGT1100.001433465910.908380511
ATAATAC5050.010.6942293
GTATATA5150.010.4870291
TAGACAG2156.4321284E-810.4662515
TGTTCGA1451.1671841E-410.34415510
TATAATA4850.09.8979812
TTCTATA4400.09.8874362
TATACTC3203.274181E-119.8448185
TACACTG2752.3592293E-99.8192465
GTCTTAT2301.7221828E-79.7840951
Produced by FastQC (version 0.11.2)