FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779765
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGG1002.572804E-615.00217830
TAGGTAG4350.013.1041715
GTATTAG5550.012.9741551
TTAGGTA5400.012.50068954
CCGTCCG1456.896771E-712.41537129
TCTATAC7100.012.0429173
CTAATAC8400.011.9649453
TAATACT12000.011.75064854
TATACTG8600.011.5122625
GTAGGAC2002.2280801E-811.2506213
TAGGACA4600.011.0875684
GTATAGA8000.011.0635091
ACTATGC8150.010.8581288
TCGCGCT1253.4268782E-410.79923511
CTATACA8650.010.4051994
CTATGCA6500.010.3838829
CTGTGCG1901.5815167E-610.2624329
CCTATCC2505.1986717E-910.20056253
CAGCGTG2803.0013325E-1010.1778529
GTATAAA9150.010.1648611
Produced by FastQC (version 0.11.2)