FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440345
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG9850.014.0114875
GTATTAG18200.012.5297361
TGGGGGG6850.012.4829415
ACCGCGC2351.2732926E-1112.1266498
CTAATAC18900.011.35028653
GTATAGA18950.011.3213061
TAGTCAG10150.011.082829
TTAGGTA14600.010.7886984
TATTAGG19050.010.70976452
TCTATAC18200.010.468033
TATACTG25500.010.4127575
TCGCCCG3350.010.29868924
TAGACTG6800.010.14802555
TATACCG11500.010.0444355
CGATCAC5700.010.00017524
GTATTAT28100.09.9840111
TCTTATA16650.09.9109322
TAATACT27800.09.8750424
GGTAGTC11850.09.8725957
TATACAG28300.09.7535795
Produced by FastQC (version 0.11.2)