FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7063978
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6200.013.7912175
TAGGGGG4300.013.6055354
GTATTAG17900.012.2365571
GCGGCCG2503.6379788E-1211.9994214
CGCGGCC2154.311005E-1011.85980813
AGGGGGG7250.011.7938695
ACCGCGC2002.2340828E-811.2493768
TGCGACG6900.010.65286430
TATACTG28300.010.4423725
CTAATAC23900.010.4190863
CTGCGCG1756.6284883E-610.2851449
GTGCGAC7150.010.28038729
GCGCGGC2803.0195224E-1010.17800712
TAGGTAG10200.010.1477175
GGTGCGA6900.010.00057828
TAATACT34750.09.8855334
CCGCGCA2003.0503488E-69.7494599
GTATAGA23700.09.7483641
TATTAGG19900.09.6488682
ATACAGG20700.09.6383086
Produced by FastQC (version 0.11.2)