FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length36
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGATGTGAGGGACTATGACTATAATGAAGGTGGA17.6923076923076925No Hit
GGATTTGATTTTATTGCAAAGGGAGAAGGAGAAATA17.6923076923076925No Hit
ATTTATTCCGTTAACGAGGGTAACTCTTACTACTGG17.6923076923076925No Hit
GGATATGAAGTATGGTTGGGGAATAACCGTGGTAAT17.6923076923076925No Hit
GTCGTGTTGCCTTCGCCAGTGTCGACATGCCGTTAC17.6923076923076925No Hit
GGCCAACTTGTACAGATTGTTTACTAGATATCTGTC17.6923076923076925No Hit
TCTTAGGATGGTGGACTTTGTTGATCAAATCTTTGG17.6923076923076925No Hit
CTTCAAATTCTTCTGAAGTGGCGGTATCAAAGTTGT17.6923076923076925No Hit
CCAGATGCTGTTTATCCCAAATATCATACCAAAAAT17.6923076923076925No Hit
CAGTTGCTCTTATCCACAAGGAAATAGACTACAGAT17.6923076923076925No Hit
ATATCATTTAGTTTCAAATAGTCGAGGTGCAGAACA17.6923076923076925No Hit
GATCATTCCAACTTTTGCATCAAGTCGTTCATTGAA17.6923076923076925No Hit
GGCTAGTAATCCACTAAATCCTTCAGCGCTTATACA17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)