FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716160
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6450.014.6523955
TAGGGGG3900.012.3087284
GTATTAG18600.011.9377331
TAGGTAG9000.011.6676475
CTAATAC22300.010.3596163
GTGCGAC7250.09.931869529
ACCGTGC4550.09.8892488
TGCGACG7300.09.65841930
GGTAGTC10400.09.5184017
CTATGCA15300.09.5089759
TTAGGTA15000.09.5007994
TAATACT35850.09.4987074
TCTATAC19700.09.2901223
TATACTG26750.09.2531155
TATTAGG19700.09.2139732
TAGGCCG2454.3292312E-79.1844465
GTATTAT33600.09.153511
TCGGTCG1654.283584E-49.09160628
CTGCGCG1654.290606E-49.0901179
CTATACT22000.09.0689454
Produced by FastQC (version 0.11.2)