FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5715352
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG4750.014.5268715
TCCGCGC1056.763116E-512.8562858
TAGGTAG7600.010.6583025
TAGGGGG3101.8189894E-1210.6455684
GTATAGA17800.010.4507511
AGGGGGG5200.010.3850135
CCTAGCG3203.6379788E-1210.31325430
CTAATAC16950.010.2658793
CGGCCGG1901.5820042E-610.26274215
GTATTAG15500.09.9689951
CCCGGAT4350.09.65520324
TATACTG23050.09.5014495
TATACGG15950.09.0285585
GCGCGCC2003.2227552E-58.99994921
CGCGCCT2003.2227552E-58.99994922
ACCGGGC2003.225079E-58.9993988
GCGTCAG4201.8189894E-128.9289134
GTATTAT30250.08.9267431
CCTAATA19850.08.7660782
GTGTAGG5000.08.7010941
Produced by FastQC (version 0.11.2)