FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7165292
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6750.013.9999315
AGGGGGG7500.012.5999385
TAGGTAG10800.012.2221625
GTATTAG20150.011.5392911
TAGTCAG10950.010.5473059
CTAATAC21100.010.1656863
TAATACT35700.09.9998824
CTATGCA16550.09.9691839
ACTATGC18650.09.8921618
TATACTG30150.09.85069755
GGTAGTC12050.09.8332917
AGGGCCG2301.7322964E-79.7825615
CGCGGCC2054.1791372E-69.51161813
TTGGGGG6500.09.4614274
TCTATAC22050.09.4556043
TATTAGG22600.09.2918612
GTCCTAG5200.09.2314331
GTATAGA20950.09.0221291
GTATAAT34750.08.9358941
GTATTAT38350.08.8793871
Produced by FastQC (version 0.11.2)