FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875789
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8250.015.4549535
TAGTCAG8150.014.9071459
GGTAGTC9000.014.3325357
TGGGGGG4900.013.4697435
GTATTAG15600.013.2702611
CGGTCGG2051.9463187E-1012.43845913
CACGCGC1652.5597728E-711.81849321
CTAATAC16600.011.7472963
CCGAGCC2701.8189894E-1211.66613714
TATTAGG15200.011.447672
TTAGGTA12950.011.351654
TCCGAGC2801.8189894E-1211.24948913
ACCTAAT15700.010.8925651
ATTAGGT14400.010.8336183
CTAGGAC3500.010.7145673
TACTATG18250.010.5199617
CCTAGCG4150.010.48231130
TCGGGGG2301.5543264E-810.43430816
TAATACT27800.010.3599854
GTAGTCA12750.010.2347238
Produced by FastQC (version 0.11.2)