FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332318
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1950.017.6935085
TAGGTAG3100.015.9688255
CCTAGCG1850.015.40678130
ACCTAAT6050.014.8786381
CTAATAC7600.014.0141093
CCTAATA7300.013.7683532
TAGTCAG3400.012.793349
GCCTCAC2600.012.692080520
GTATTAG6450.012.5603381
TATTAGG4900.012.5521422
TAGGGGG1501.0396507E-612.0008144
CTATGCA7000.011.999279
TAATACT11250.011.8674734
ACCTAGC2259.131327E-1011.33410429
ATCACAC6500.011.30845929
CTAGGTA1355.6490986E-511.1118654
TCACACA6500.011.07791230
ACTATGC8600.010.9877058
TTAGGTA4650.010.9684864
CTAGACC2751.8189894E-1110.9098324
Produced by FastQC (version 0.11.2)