FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528546
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGA32370.12801823656757677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG2650.014.1502439
CTCTTAT6750.014.0015241
CTATGCA4300.013.9527999
TAGGTAG2950.013.2209935
GTATTAG5300.012.7372341
GTCCTAG1201.6932609E-512.501361
CTAATAC6500.012.4619083
TTATCCT6350.012.0478394
CTAGCCT2900.011.89714054
TAATACT9700.011.7531594
GTAGAGG8700.011.72564529
AATACTA7850.011.6566275
TCTTATA9350.011.55137352
ATCACAC4200.011.43004129
TATACTG6500.011.3082515
ATACTAT7450.011.2757256
TACTATG6300.011.1899227
CACTCCG3100.011.128262514
ACTATGC7450.011.0732778
TAACACT3950.011.0132044
Produced by FastQC (version 0.11.2)