FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360066
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10950.014.7962395
GTATTAG18150.013.2254041
TAGTCAG10900.013.2101559
CGCGCCT3150.012.85671422
GGTAGTC11000.012.1354837
ATCACAC14950.011.63968929
TATTAGG19900.011.4584372
CTATGCA17850.011.3438039
TCACACA15750.010.85806230
GTCGGGG3051.8189894E-1210.81880215
TGGGGGG4450.010.7877685
CTAATAC23650.010.7833233
CCTAATA22800.010.6589652
CCTACAC5100.010.5892983
TTAGGTA16900.010.5633564
ACTATGC23300.010.5572578
ACCTAAT21550.010.2337691
CGGTCGG3550.010.14002913
CTAGGAC4450.010.1133753
TCTATAC19050.09.92225553
Produced by FastQC (version 0.11.2)