FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000376
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2401.8189894E-1212.5010415
CTAATAC8000.012.1885173
GTATTAG7600.011.6470661
ACCCGCG950.006001906511.05226323
CGCACTC2251.1030352E-810.66613412
ACCGTGC1551.9578663E-510.6446298
CGCCCGC1000.00878260910.50000125
TAACCCG1304.9154926E-410.3854815
TAGGTAG4900.010.1028835
CCTATAC6100.010.0828073
CCTAGAC3152.3646862E-1110.00083353
GAGCGAC1356.965287E-49.999511
TAGGACC3101.8553692E-109.67822554
GGGGGTG1552.2869982E-49.67822555
GGGTGCG1409.72343E-49.6423757
CCAGCGC3001.0531949E-99.4995258
ACTATGC7900.09.49319658
CTATGCA7300.09.4515829
TAATACT14550.09.3822254
TCCTATA6150.09.2690652
Produced by FastQC (version 0.11.2)