FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503957
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGC601.1852535E-825.00463329
CTACGCT603.273508E-722.5041730
CCCCGAT402.4667187E-422.4974699
GGCTCCT350.003000611721.4325427
TAGGTAG751.26347E-719.9977515
TCGGGGG455.4648524E-419.99775116
TAGTCAG751.26347E-719.9977519
CAGCTCC400.00650976518.747894
CCCCAGA500.001109145817.9997621
TAGTCCT500.001109884717.9979764
CTATGCA1303.45608E-1117.3057469
GTCTACT703.107172E-517.142631
TAGCACT703.10982E-517.140934
ACGCTAC805.1523966E-616.87812627
CTCCGAG805.167023E-616.87310212
TCCGAGC805.167023E-616.87310213
AGCCCAC908.568986E-716.66479117
AGCGGGA550.002101018316.36179710
TCTCAGC550.002101018316.3617978
TAGGACA550.002101018316.3617974
Produced by FastQC (version 0.11.2)