FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362273
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1950.016.154445
GTCGGGG755.627382E-515.99823815
CCTAATA3500.015.8577252
GCCGTGC706.0429226E-414.9983488
ACCTAAT2950.014.7468461
GTATTAG3450.014.348881
TAGTCAG2200.014.3166059
GCGAGTC952.6385815E-514.20896116
TTAGGTA2450.014.08214954
CTATGCA3150.013.8080039
TATTAGG2900.013.448772
TCGGGGG902.6549833E-413.33186516
TCTAAGC2500.013.2004833
TCCGAGC1855.0749804E-1012.97154513
GGTAGTC2600.012.6909097
CGGTCGG954.1921964E-412.63018713
ATTAGGT3500.012.4290283
CCGAGCC1857.032213E-912.16082314
TCAGGGG1006.448329E-412.0004413
CGCTTGC1252.5773637E-511.99867813
Produced by FastQC (version 0.11.2)