FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length36
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTAACAACATCTTGACTGTAATGATAAAGGTTA15.0No Hit
GAGCAGCTGTGGTTCAGCACCATTAAATGATCACGA15.0No Hit
ATAGAGATATGAATAAGAAATAAAAAATAAAAAAAG15.0No Hit
GCATTAGTGTTGGTCAAAGAATTGGAAATTCTTAAA15.0No Hit
GGATTGAACCACTATGAAACGTTTGAAGCGTTGATG15.0No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG15.0No Hit
GACGAAATTCAATAATGCCCACAATTCTGGTAAATT15.0No Hit
GGCAGTAATCAATGGAACCTTTTCATAGACACACTC15.0No Hit
GGTTTCAACAGAAGTACCAGTCTCGGAAGAAGAATC15.0No Hit
TAATTATGCAGGAGAGCCACACCAAATGCGTCAGCG15.0No Hit
AAGCTCGCTTATGTAATCGAAATGAAAGTAGTTGCA15.0No Hit
TTTATACACTATCTACTGGAGAAGTGAAACAAATAA15.0No Hit
AAATAGATTAAGTCAAAGGAAAAATCATGTCGATGT15.0No Hit
CCAATATACCTTTCTATAGTAGTAACTTCATCGAAC15.0No Hit
CTTTTGATAGGTGGACTGACATACAGCCTGACATTC15.0No Hit
GTCCAATTCATTTCTCAAGAATTGTGACTTTTCTTC15.0No Hit
AACAAGTGCAGGGGAAGAAGAGTCTGATTCTCAACC15.0No Hit
GGATTGGAGTGTGAGAGTAGGGTAAGTTTGAGAGTG15.0No Hit
ATAAATATGTAGAGATTATGATCTGTAGATCTCTGA15.0No Hit
GGTTGGCACTCTGCACCCCTAAATTTAAGGCTGTTT15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)