FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6744342
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19400.015.3880971
GGTAGTC12900.015.3482847
TAGGTAG14250.015.0532055
TAGTCAG12650.014.821499
CCTAGCG6900.014.13097330
CTATGCA16950.013.6277269
CTAATAC23150.013.2833443
TATTAGG18850.013.0507972
TTAGGTA17200.012.994684
CCTAATA22200.012.3652432
CGATCAC7650.012.15687524
TAATACT36150.011.9506634
ATCACAC16200.011.9448129
ACTATGC23750.011.8100138
ATTAGGT18900.011.7463913
CTAGGCA8900.011.62870810
ACCTAGC8550.011.57930229
ACCTAAT21000.011.3582491
TCACACA17050.011.34940630
GTATAGA23900.011.2981711
Produced by FastQC (version 0.11.2)