FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026301
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12700.016.182065
GGTAGTC13150.014.3716847
TAGTCAG13000.013.9607139
GTATTAG23000.013.3062121
CTATGCA21850.012.0816659
CGATCAC7600.012.03943524
CGCCGCG8550.011.92978724
CCTAATA27150.011.8790082
CTAATAC28300.011.8733463
CCTAGCG7650.011.76584230
TTAGGTA19250.011.6890034
ATCACAC18050.011.635429
TAGGGGG4950.011.5158334
GCCGCGT8800.011.4211325
ACCTAAT23500.011.2356181
TAATACT43500.011.0351364
ACTATGC26550.011.0162978
TATTAGG23350.010.9855242
ACCTAGC8750.010.45808829
TGGGGGG8650.010.405245
Produced by FastQC (version 0.11.2)