FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032502
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC11050.015.7454017
TAGGTAG12300.015.0004685
GTATTAG19250.014.8848151
TAGTCAG11400.013.9464189
ATCACAC13900.013.92210629
CCTAGCG4950.013.03146630
CTATGCA16250.012.92219459
CTAATAC21150.012.8371733
TCACACA15450.012.42829530
TATTAGG20850.012.23072
TTAGGTA18750.011.9203734
ACTATGC20750.011.8546138
GGCGCCG2950.011.695765525
CCTAATA22750.011.1432972
TAATACT35150.011.0956534
AGTCAGA16300.011.04219110
TGGGGGG4350.011.0348275
ATTAGGT19650.010.9926193
CGATCAC5750.010.95686424
GACATAG15450.010.87475830
Produced by FastQC (version 0.11.2)