FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6708617
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG11250.015.4678095
GTATTAG20050.013.0196791
TAGTCAG10850.012.8564629
GGTAGTC11650.012.230937
TGGGGGG6650.011.5046085
CTAATAC23900.011.4232663
CCTAATA23050.011.3890422
TTAGGTA17700.011.1872664
TATTAGG21650.011.0168972
CTATGCA17550.010.9395919
ACTATGC22000.010.8403368
CGGGTCC4050.010.740972525
TAATACT34650.010.6501374
ATCACAC15300.010.29472429
ACCTAAT20300.010.272691
GCGCGGT1901.5824098E-610.26261411
CCTAGCG5000.010.20060130
CCGGATC7950.010.18889925
CTTAGAC9900.010.0005893
GGGGCTC3600.09.99947110
Produced by FastQC (version 0.11.2)