FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6808953
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG6400.012.65704255
GTATTAG21050.011.8306751
CGCGCAT2351.546141E-1011.48856410
TAGGTAG12050.011.4529995
GGTAGTC11300.011.2824027
TAGTCAG10800.011.1103399
CTAATAC19700.010.43197253
CGCCGCG3203.6379788E-1210.31261524
GCCGCGT3603.6379788E-129.58393425
GACCGCG2054.179492E-69.5115347
TACGGTG16400.09.5115347
TCTATAC21950.09.4993173
ACTATGC18650.09.4899568
CGACCGA1901.7607797E-59.47302510
TAATACT35650.09.4675654
TTAGGTA19750.09.3422894
ACCGCGC2105.6782173E-69.2850698
CTATGCA16900.09.2301279
ACGGTGT16750.09.2232398
CCTAGCG5050.09.20870130
Produced by FastQC (version 0.11.2)