FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6221661
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG5450.011.8355955
CTACGCG1059.726759E-411.4278099
GTATTAG15550.011.0950051
CTAATAC18100.09.8624893
AGTCGCG1409.726589E-49.64236820
TATACAG30050.09.634545
TAGACAG9600.09.3755815
CTATGCA13900.09.2799569
GTATATA49650.09.2763931
TAATACT29350.09.1998874
GGTGCGA6300.09.04832528
TATACTG22900.09.0398615
GTCCTAA7500.09.0014251
ACTATGC15300.08.9209748
CGTGCGC2201.0237029E-58.86304610
TACTATG20800.08.725387
GTATTAA18450.08.7005641
TAGGTAG8700.08.6212235
TCTTATA18850.08.5151162
CCTATAC11100.08.5140413
Produced by FastQC (version 0.11.2)