FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_combined_n01_ntr_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_combined_n01_ntr_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7431758
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19150.011.9077491
TAGGTAG12450.011.2050285
CTGTCCG3401.8189894E-1210.1464289
TCTATAC22450.09.7551933
TAGTCAG10550.09.6676469
CTGTGCG4550.09.5598459
TATTAGG21100.09.45515252
GGTAGTC11250.09.3326917
TATACTG27500.09.1092615
GTATATA55700.09.0497991
CCGTGCG2003.2253098E-58.9994419
ACAGTGC10300.08.8829438
GTATTAA25300.08.8352861
CTATACT22200.08.7839474
GTATAGA21200.08.774871
GACGCGC3254.469257E-98.7686868
CGCGGCC2403.2384705E-68.74957413
TATACAG36850.08.6704465
CTAATAC21800.08.6698873
GTCCGAC2105.7192163E-58.57089611
Produced by FastQC (version 0.11.2)