##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_combined_n01_ntr_11.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27 Sequences flagged as poor quality 0 Sequence length 36 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.925925925925927 0.0 0.0 0.0 0.0 0.0 2 31.333333333333332 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 31.333333333333332 0.0 0.0 0.0 0.0 0.0 5 31.40740740740741 0.0 0.0 0.0 0.0 0.0 6 34.7037037037037 0.0 0.0 0.0 0.0 0.0 7 34.888888888888886 0.0 0.0 0.0 0.0 0.0 8 31.77777777777778 0.0 0.0 0.0 0.0 0.0 9 35.7037037037037 0.0 0.0 0.0 0.0 0.0 10 35.03703703703704 0.0 0.0 0.0 0.0 0.0 11 35.18518518518518 0.0 0.0 0.0 0.0 0.0 12 32.2962962962963 0.0 0.0 0.0 0.0 0.0 13 34.888888888888886 0.0 0.0 0.0 0.0 0.0 14 35.55555555555556 0.0 0.0 0.0 0.0 0.0 15 33.55555555555556 0.0 0.0 0.0 0.0 0.0 16 32.96296296296296 0.0 0.0 0.0 0.0 0.0 17 33.925925925925924 0.0 0.0 0.0 0.0 0.0 18 34.888888888888886 0.0 0.0 0.0 0.0 0.0 19 34.22222222222222 0.0 0.0 0.0 0.0 0.0 20 34.55555555555556 0.0 0.0 0.0 0.0 0.0 21 34.592592592592595 0.0 0.0 0.0 0.0 0.0 22 33.48148148148148 0.0 0.0 0.0 0.0 0.0 23 31.77777777777778 0.0 0.0 0.0 0.0 0.0 24 33.407407407407405 0.0 0.0 0.0 0.0 0.0 25 35.55555555555556 0.0 0.0 0.0 0.0 0.0 26 35.25925925925926 0.0 0.0 0.0 0.0 0.0 27 33.925925925925924 0.0 0.0 0.0 0.0 0.0 28 34.074074074074076 0.0 0.0 0.0 0.0 0.0 29 33.74074074074074 0.0 0.0 0.0 0.0 0.0 30 34.22222222222222 0.0 0.0 0.0 0.0 0.0 31 32.592592592592595 0.0 0.0 0.0 0.0 0.0 32 33.629629629629626 0.0 0.0 0.0 0.0 0.0 33 33.77777777777778 0.0 0.0 0.0 0.0 0.0 34 33.25925925925926 0.0 0.0 0.0 0.0 0.0 35 33.51851851851852 0.0 0.0 0.0 0.0 0.0 36 34.074074074074076 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 12508 1 0.0 12508 2 0.0 12508 3 0.0 12508 4 0.0 12508 5 0.0 12508 6 0.0 12508 7 11.0 12508 8 2.0 12508 9 0.0 12508 10 0.0 12508 11 0.0 12508 12 0.0 12508 13 2.0 12508 14 0.0 12508 15 0.0 12508 16 0.0 12508 17 11.0 12508 18 0.0 12508 19 0.0 12508 20 0.0 12508 21 0.0 12508 22 0.0 12508 23 11.0 12508 24 0.0 12508 25 0.0 12508 26 2.0 12508 27 0.0 12508 28 2.0 12508 29 0.0 12508 30 -2.0 12508 31 0.0 12508 32 0.0 12508 33 0.0 12508 34 0.0 12508 35 7.5 12508 36 -2.0 12210 1 0.0 12210 2 0.0 12210 3 0.0 12210 4 0.0 12210 5 0.0 12210 6 0.0 12210 7 -11.0 12210 8 -2.0 12210 9 0.0 12210 10 0.0 12210 11 0.0 12210 12 0.0 12210 13 -2.0 12210 14 0.0 12210 15 0.0 12210 16 0.0 12210 17 -11.0 12210 18 0.0 12210 19 0.0 12210 20 0.0 12210 21 0.0 12210 22 0.0 12210 23 -11.0 12210 24 0.0 12210 25 0.0 12210 26 -2.0 12210 27 0.0 12210 28 -2.0 12210 29 0.0 12210 30 2.0 12210 31 0.0 12210 32 0.0 12210 33 0.0 12210 34 0.0 12210 35 -7.5 12210 36 2.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 2.0 31 1.0 32 4.0 33 8.0 34 7.0 35 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.44444444444444 14.814814814814813 25.925925925925924 14.814814814814813 2 14.814814814814813 33.33333333333333 37.03703703703704 14.814814814814813 3 14.814814814814813 37.03703703703704 29.629629629629626 18.51851851851852 4 0.0 18.51851851851852 62.96296296296296 18.51851851851852 5 3.7037037037037033 59.25925925925925 22.22222222222222 14.814814814814813 6 11.11111111111111 44.44444444444444 22.22222222222222 22.22222222222222 7 29.629629629629626 40.74074074074074 22.22222222222222 7.4074074074074066 8 14.814814814814813 51.85185185185185 11.11111111111111 22.22222222222222 9 14.814814814814813 14.814814814814813 33.33333333333333 37.03703703703704 10 14.814814814814813 25.925925925925924 33.33333333333333 25.925925925925924 11 29.629629629629626 18.51851851851852 33.33333333333333 18.51851851851852 12 11.11111111111111 29.629629629629626 40.74074074074074 18.51851851851852 13 18.51851851851852 29.629629629629626 37.03703703703704 14.814814814814813 14 18.51851851851852 22.22222222222222 33.33333333333333 25.925925925925924 15 25.925925925925924 29.629629629629626 33.33333333333333 11.11111111111111 16 18.51851851851852 33.33333333333333 33.33333333333333 14.814814814814813 17 18.51851851851852 25.925925925925924 25.925925925925924 29.629629629629626 18 18.51851851851852 40.74074074074074 37.03703703703704 3.7037037037037033 19 29.629629629629626 18.51851851851852 22.22222222222222 29.629629629629626 20 40.74074074074074 22.22222222222222 22.22222222222222 14.814814814814813 21 18.51851851851852 25.925925925925924 44.44444444444444 11.11111111111111 22 11.11111111111111 33.33333333333333 40.74074074074074 14.814814814814813 23 33.33333333333333 29.629629629629626 11.11111111111111 25.925925925925924 24 14.814814814814813 37.03703703703704 29.629629629629626 18.51851851851852 25 14.814814814814813 25.925925925925924 25.925925925925924 33.33333333333333 26 25.925925925925924 18.51851851851852 44.44444444444444 11.11111111111111 27 29.629629629629626 14.814814814814813 40.74074074074074 14.814814814814813 28 22.22222222222222 25.925925925925924 29.629629629629626 22.22222222222222 29 18.51851851851852 29.629629629629626 29.629629629629626 22.22222222222222 30 14.814814814814813 33.33333333333333 22.22222222222222 29.629629629629626 31 18.51851851851852 44.44444444444444 18.51851851851852 18.51851851851852 32 3.7037037037037033 40.74074074074074 29.629629629629626 25.925925925925924 33 11.11111111111111 40.74074074074074 37.03703703703704 11.11111111111111 34 11.11111111111111 25.925925925925924 48.148148148148145 14.814814814814813 35 18.51851851851852 40.74074074074074 18.51851851851852 22.22222222222222 36 14.814814814814813 18.51851851851852 55.55555555555556 11.11111111111111 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.5 25 3.0 26 2.5 27 2.0 28 2.0 29 1.5 30 1.0 31 1.0 32 2.5 33 4.0 34 4.0 35 3.5 36 3.0 37 3.0 38 3.0 39 3.0 40 3.5 41 4.0 42 4.0 43 4.5 44 5.0 45 5.0 46 2.5 47 0.0 48 0.0 49 0.0 50 0.0 51 0.5 52 1.0 53 1.0 54 0.5 55 0.0 56 0.0 57 0.5 58 1.0 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 27.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAATATCCAGTACGGGTGGTACCAACAACAAAACT 1 3.7037037037037033 No Hit TAATAATATCGACCTCCTCCTACCTACCTATCTACC 1 3.7037037037037033 No Hit GTCCAATATACTTTGGATCCCTCCATGGACCGATAT 1 3.7037037037037033 No Hit TATTCATACATTGAATAACGCAGTTCTTGAGTTAGG 1 3.7037037037037033 No Hit GGGTACGGCCCATTCTGTGGAGGTGGTACTGAAGCA 1 3.7037037037037033 No Hit GTATATAACGAAATATGGTGGTTCTTTATTTATCAC 1 3.7037037037037033 No Hit AAACTCGACCTCTACATTTTTTATGTTTATCTCTAG 1 3.7037037037037033 No Hit ATGCACAGTTTCTCTAGAAGTTGAAGCGGACAATGA 1 3.7037037037037033 No Hit GTATATGATTGTACATCTGTTAAGTATATAATATGC 1 3.7037037037037033 No Hit TAATTACAGTATCGTTCGTCCCGACTTGGCATTAGT 1 3.7037037037037033 No Hit GTATAAGCAATTTATACAGTGAAACTGCGAATGGCT 1 3.7037037037037033 No Hit GATTTTATTATTGATACAAAGCGCTATGAAGATCAA 1 3.7037037037037033 No Hit GGCTGAAAGCTTATTTAAAAATCACTAATGAAATAA 1 3.7037037037037033 No Hit AAATAAATACAATGGTATCGATAACGGTAAAATTCT 1 3.7037037037037033 No Hit TCTTTAGGAGTTACTACAGGGTGTAGCTTGGCGTAT 1 3.7037037037037033 No Hit GGTCCTGATGTATTGTATGATCGAATGTTGTAATTT 1 3.7037037037037033 No Hit TAATTCACCTACTGGCTACTATTGCGTTCCACATTT 1 3.7037037037037033 No Hit CTCAATAGCTGAGTTCTTCCTAAACGAGTAAAATCA 1 3.7037037037037033 No Hit GTATCGCATCCACTAGCGGAATATGTTCACCTATAG 1 3.7037037037037033 No Hit GCCTACAAGGGCATGCACGAAAAGCCTCATACGGAT 1 3.7037037037037033 No Hit GATACAACCTCGATAGTGCTTTCTTTGTCCCATTTT 1 3.7037037037037033 No Hit TCTTAGGCCTGTAGAATGAGGCAGTAGCACATTCTT 1 3.7037037037037033 No Hit TATTAAGATCAATAGGCACGTGCTATTTTCGAACAT 1 3.7037037037037033 No Hit CTGAAATCTACCTCCAATTGTGTACGGACTATTATT 1 3.7037037037037033 No Hit GGTTACTAATGGGCAAGAAGAAGAGTAAGAACCAAT 1 3.7037037037037033 No Hit CTCCATATGTGTGAATTATTTTGTCCGGCGTCTTGT 1 3.7037037037037033 No Hit ATGAAGAACATGCGTATATATACAATACCTTCCAAG 1 3.7037037037037033 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE