FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_96.35200000039e6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_96.35200000039e6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677103
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT40880.6037486172709322No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC31900.471124777175703No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC29580.4368611570174701No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC25190.37202611714908956No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC24390.36021107571521616No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG23070.340716257349325No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG21620.3193014947504294No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC20080.29655753999022305No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT19640.2900592672015927No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT18780.2773580976601787No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT18310.2704167608177781No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG18050.2665768723517692No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG15110.22315659508228436No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT13950.2060247850031679No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13840.2044002168060103No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG13800.20380946473431663No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC13570.20041264032207803No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT13560.20026495230415464No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12760.18844991087028118No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG12500.18461002240427232No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT12440.1837238942967318No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG12300.18165626204580396No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11870.175305677275097No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA11760.1736811090779394No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC11480.1695458445760837No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG11350.16762590034307925No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC11350.16762590034307925No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC10980.1621614436799128No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10890.16083225151860206No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA10700.1580261791780571No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT10220.15093715431773305No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC9950.14694957783380078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9790.1445865695470261No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC9540.14089436909894062No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC9350.13808829675839568No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT9310.13749754468670203No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG9310.13749754468670203No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9110.13454378432823366No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8940.13203308802353558No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC8920.13173771198768872No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT8890.13129464793391848No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8770.12952239171883745No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8610.12715938343206276No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8580.12671631937829253No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC8450.1247963751452881No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8060.11903654244627478No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG8030.11859347839250453No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT7940.11726428623119378No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA7810.11534434199818935No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7680.11342439776518491No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7640.11283364569349125No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA7550.11150445353218048No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC7520.11106138947841024No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA7490.11061832542463997No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7470.11032294938879315No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA7470.11032294938879315No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7450.1100275733529463No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7440.10987988533502288No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7390.1091414452454058No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA7380.10899375722748236No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7380.10899375722748236No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7190.10618768488693743No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT7170.1058923088510906No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7100.10485849272562668No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6790.10028016417000071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCT2500.026.3971425
CCCGTGC2550.025.8795494
ACCCGTG2550.025.8795493
TCTCTTA452.0337477E-523.34114830
GTACCCG2850.023.1570971
TATGCGC5150.023.00721511
ACTCGGT1450.022.7561553
CCGTATG8100.022.5901478
ACCGTAT8250.022.1794157
CCACCGT8350.022.0934145
GTATGCG8300.022.04580510
GTGTTAG3000.022.0073728
TGTTAGG3000.022.0073729
CTCGGTC1500.021.997624
TGTGTTA2950.021.8717327
CTTGCAT2900.021.72178521
GTTAGGC3050.021.64659130
ATCCACC8550.021.5766093
CACCGTA8550.021.5766096
ATGCGCA5450.021.46556312
Produced by FastQC (version 0.11.2)