FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_95.35200000039e36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_95.35200000039e36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931556
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT73630.7903980007643125No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC52020.5584205351047066No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC39170.4204792841224789No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC38350.4116768074061033No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG32170.34533619020219936No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG29430.3159230362962613No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC27730.29767399920133625No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT27040.2902670370863373No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC26210.28135721309293266No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG25980.2788882257212663No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG25900.2780294475050346No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT24410.2620347032277179No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT24170.25945836857902266No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG22640.24303423519359008No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG20650.22167212706482486No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC20340.2183443614769268No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG20020.21490924861199973No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT19990.2145872067809128No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC19710.21158148302410162No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA19670.21115209391598572No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT18990.2038524790780157No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC18840.20224226992258115No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT18810.20192022809149424No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG18280.19623082240895878No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC17970.19290305682106068No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT17750.1905414167264233No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC17070.1832418018884533No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG16690.1791626053613524No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT16540.17755239620591784No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG16430.17637157615859916No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT15090.16198704103671707No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT15060.16166499920563016No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC14990.16091356826642733No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA14840.1593033591109928No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14530.15597559352309467No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT14170.15211109155005173No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT14110.15146700788787792No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG14020.15050088239461717No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC13600.1459922967594004No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC13330.1430939202796182No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA13260.1423424893404154No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA12580.13504287450244537No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC12550.13472083267135845No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC12490.13407674900918462No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC12450.13364735990106874No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC12380.13289592896186594No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA12350.13257388713077903No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG12270.13171510891454727No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG12260.1316077616375183No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT12030.12913877426585196No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA11760.12624039778606977No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA11400.1223758958130268No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT11330.121624464873824No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT11310.12140977031976606No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC11000.11808200473186797No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC10880.11679383740752032No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC10740.11529097552911473No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT10600.11378811365070914No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC10510.1128219881574484No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA10450.11217790449527458No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT10440.1120705572182456No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG10330.11088973717092693No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA10230.1098162644006372No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA10230.1098162644006372No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG10060.10799136069114472No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC10050.10788401341411573No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT10010.10745462430599986No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA9910.10638115153571014No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG9690.10401951144107278No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG9530.10230195500860925No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC9490.10187256590049336No Hit
GTCGTTACTCATGTCAGCATTCGCACTTCTGATACC9360.10047705129911674No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT9320.10004766219100086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGG254.2217798E-430.0047829
ACCCGTG5050.026.7283213
CCCGTGC5100.026.4662784
GTACCCG5250.025.9971621
CTCGGTC2250.025.9957684
ACTCGGT2250.025.9957683
TGAATTC300.001226009225.00129720
GTTAGGC5500.024.82213630
TGTGTTA5300.024.62656427
GTGTTAG5250.024.57534228
TGTTAGG5300.024.343529
CATGTGT5450.024.22404325
ATGTGTT5400.024.17051726
CCTATGA501.696304E-624.00124516
CCGTGCT5600.023.8354035
CTTGCAT5750.023.74036221
GACTTGC5700.023.42226819
CGACTTG5650.023.36404418
TGCATGT5600.023.30478123
TTGCATG5950.022.94236822
Produced by FastQC (version 0.11.2)