FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_94.35200000039df1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_94.35200000039df1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660865
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT50690.7670250353703102No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC25110.3799565720684255No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC22460.33985761085849603No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG19460.2944625604321609No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG19040.28810725337247395No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG15660.23696216322546967No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT14860.22485681644511363No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT14450.21865282622018112No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA14120.21365937067328425No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC14070.21290278649951203No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG13960.21123830131721302No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG13660.20669879627457952No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT13480.2039750932489994No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT13400.20276455857096382No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG13230.20019217238013814No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13080.1979224198588214No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12370.1871789245912554No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG11660.1764354293236894No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT11200.16947485492498468No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC11190.16932353809023024No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC11120.16826432024694907No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT11110.16811300341219462No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG10790.1632708647000522No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC10580.16009321117020875No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC10560.15979057750069983No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC10470.15842872598790977No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10450.15812609231840088No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT9880.1495010327373972No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA9450.14299440884295583No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9340.14132992366065686No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8980.13588251760949666No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8830.13361276508817987No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG8760.13255354724489873No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC8760.13255354724489873No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC8710.13179696307112646No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8390.12695482435898406No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC8340.1261982401852118No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG8300.125592972846194No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8170.12362585399438614No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8090.12241531931635054No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT7870.11908634895175263No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7810.11817844794322592No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT7720.11681659643043586No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7600.11500079441338247No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7570.11454684390911911No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC7530.1139415765701013No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7510.11363894290059241No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC7460.11288235872682015No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7340.11106655670976674No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC7250.10970470519697668No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT7210.10909943785795889No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7200.10894812102320445No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7180.10864548735369554No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7150.10819153684943218No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG7140.10804022001467772No Hit
GTCGTTACTCATGTCAGCATTCGCACTTCTGATACC7130.10788890317992329No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC6780.1025928139635175No Hit
CTTGTAGGTATTCGGAGTTTGCATCGGTTTGGTAAG6750.10213886345925416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCT200.00514823329.99697123
GGGTAAT300.00122672424.9974781
TACCTAG652.7761416E-823.08158127
TATGCGC3250.023.07634211
TAGGATA603.2881508E-722.4960274
CCTAGAT706.084883E-821.43289829
ACCTAGA706.084883E-821.43289828
CTTGTGG504.5916815E-521.00423824
GTAGGAT657.1033355E-720.7655643
CATTCGT2350.020.42656321
GCCATAC9200.020.0523221
ATTCGTG2400.019.99798222
CATACCC9250.019.780293
ATCGTTA2200.019.77222811
CACCGTA5100.019.7038966
GTACCCG1450.019.6531891
CCATACC9250.019.6181562
CCACCGT5050.019.60055
ACCGTAT5150.019.5125947
TACCCTT9250.019.4560245
Produced by FastQC (version 0.11.2)