FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_93.35200000039dbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_93.35200000039dbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73553
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3320.45137519883621335No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC2800.3806778785365655No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2720.36980136772123506No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2240.3045423028292524No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2120.2882275366062567No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1780.24200236564110236No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1600.21753021630660885No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1570.2134515247508599No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1420.19305806697211533No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1380.18761981156445012No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1350.1835411200087012No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT1270.17266460919337076No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1240.16858591763762185No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1220.16586678993378923No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1190.1617880983780403No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1160.1577094068222914No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC1130.1536307152665425No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG1070.14547333215504465No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1070.14547333215504465No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG1060.14411376830312836No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC970.13187769363588162No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT940.1277990020801327No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC940.1277990020801327No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC920.12507987437630008No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA910.12372031052438379No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC900.12236074667246748No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC870.11828205511671855No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT860.11692249126480224No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC840.11420336356096963No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG830.11284379970905334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.11284379970905334No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG790.10740554430138811No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC790.10740554430138811No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA780.10604598044947182No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT770.10468641659755551No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT750.10196728889372289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC200.00511635829.99999818
GGGTCTA200.00511635829.9999986
GTGTTAG200.00511635829.99999828
CCAGCTA200.00511635829.99999824
ACTTGCA200.00511635829.99999820
GCTATCT200.00511635829.99999827
CGGGTCT200.00511635829.9999985
TATCTAG200.00511635829.99999829
ACGGACC200.00511635829.99999827
CTATCTA200.00511635829.99999828
ACTGTCC359.85165E-525.7142858
TGTCCTC300.001215669325.010
TATGCGC300.001215669325.011
ACACTGT300.001215669325.06
TACACTG300.001215669325.05
ATAACGG300.001215669325.024
CTGTCCT300.001215669325.09
ATACACT300.001215669325.04
CATACAC300.001215669325.03
CGTACAA452.007196E-523.33333215
Produced by FastQC (version 0.11.2)