FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_92.35200000039d7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_92.35200000039d7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133691
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT6800.5086355850431218No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4580.34258102639669086No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4080.3051813510258731No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3210.24010591588065014No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG3050.22813801976198847No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.22215407170265764No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2930.2191620976729922No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2630.19672229245050155No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2550.19073834439117068No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2490.18625038334667254No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2370.1772744612576763No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2270.16979452618351273No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG2130.15932261707968376No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2090.15633064305001831No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2080.15558264954260195No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC2080.15558264954260195No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2050.1533386690203529No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1950.14585873394618934No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1880.14062277939427484No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1850.13837879887202578No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1820.13613481834977673No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1780.13314284432011128No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1750.13089886379786222No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1750.13089886379786222No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1710.1279068897681968No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1680.12566290924594772No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1680.12566290924594772No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1660.12416692223111503No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1660.12416692223111503No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1660.12416692223111503No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1640.12267093521628233No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1630.12192294170886597No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1520.11369501312728605No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC1490.11145103260503698No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1470.10995504559020428No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1460.10920705208278791No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG1430.10696307156053886No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT1400.10471909103828979No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1360.10172711700862436No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1360.10172711700862436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTA254.2084197E-429.99775521
CTCAAAG303.4698158E-529.99775311
TTAATTC200.005142465229.9865323
TAATTCG200.005142465229.9865324
CGAAGCA254.2176625E-429.986539
CAAAGTT359.916008E-525.7123613
CCTCAAA359.9412864E-525.70274210
CTTAGGT300.001216095925.01685324
CTGAGAT300.00121875625.00748817
AGATCTT300.001221420724.99812720
ATCTTAG300.001221420724.99812722
GTTAGAA300.001224090324.9887773
TTTCGGG402.4591803E-422.48992
GTGGTAT350.002980739621.44301826
TCTTAGG350.002987227921.4349923
GCTGAGA350.002993727621.42696816
GAGATCT350.002993727621.42696819
TAGGTGG350.00300023921.4189514
TAGAACC350.00300023921.4189515
AACCTCA350.00300023921.4189518
Produced by FastQC (version 0.11.2)