FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_91.35200000039d37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_91.35200000039d37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118922
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT10140.8526597265434487No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4780.4019441314475034No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4770.4011032441432199No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3680.3094465279763206No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC3410.28674257076066667No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3030.2547888531978944No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2930.24637998015505963No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG2890.2430164309379257No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2720.22872134676510653No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2620.22031247372227172No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2570.21610803720085434No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2510.21106271337515345No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2470.20769916415801953No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2410.20265384033231865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2320.19508585459376734No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2290.1925631926809169No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG2200.18499520694236557No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA2180.18331343233379863No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1930.1622912497267116No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1850.1555641512924438No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1840.1547232639881603No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1830.15388237668387683No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1740.1463143909453255No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1730.14547350364104203No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1710.14379172903247506No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1590.1337010813810733No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1550.13033753216393937No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1510.12697398294680548No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1480.12445132103395502No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1470.12361043372967155No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1310.11015623686113586No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1300.10931534955685239No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1290.1084744622525689No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1270.10679268764400196No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC1260.10595180033971846No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1260.10595180033971846No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT1260.10595180033971846No Hit
GTGATACATGAGGCGCTACCTAAATAGCTTTCGAGG1250.10511091303543499No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1250.10511091303543499No Hit
GCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAA1240.10427002573115152No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1220.10258825112258456No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT1210.10174736381830106No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1210.10174736381830106No Hit
GGCCGACTCGACTAGTGAGCTATTACGCTTTCTTTA1200.10090647651401759No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT1190.10006558920973412No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG1190.10006558920973412No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC1190.10006558920973412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAA200.005123302330.00673317
GGATACA254.1982837E-430.00673129
TCGCCTC200.00513385829.9941124
CTATAGT200.00513385829.99411214
GCTATAG200.00513385829.99411213
TATAGTA200.00513385829.99411215
ATAGTAA200.00513385829.99411216
AAGCTAT200.00513385829.99411211
CACCGTA850.026.4653936
CCACCGT850.026.4653935
TATGCGC708.185452E-1125.70923811
GTGGGGA300.001218498625.0056126
TCTAGCC300.001218498625.0056120
CCGTCTA300.001218498625.0056117
GCCGCGG300.001218498625.0056124
GCGGATA300.001218498625.0056127
CCGCGGA300.001218498625.0056125
TCTTTCC300.00122149324.99509412
ACCGTAT900.024.9950927
CCGTATG950.023.6795628
Produced by FastQC (version 0.11.2)