FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_89.35200000039cbe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_89.35200000039cbe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701820
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9150.13037530990852356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG1100.021.81797410
CCCGGAG1400.021.42836817
GCCCGGG1450.020.69535825
CGGCCCG1450.020.68945914
GGAGCCC1250.020.3983528
CCCGCTG455.4639945E-419.99981112
CCCGGGG1400.019.2910326
CCCGGCC1650.019.09072712
GAGCCCG1350.018.888719
AGGAGCC1450.018.6191867
GCCCGGC1650.018.18164411
CCGGCCC1700.017.64689313
CCGGAGG1700.017.64563418
CCGGGGC1550.017.42415627
GCCCGGA1750.017.14269416
GGGCCCG1850.017.02565223
CGGAGGG1800.016.66532119
AAGGAGC1700.015.881076
GGAGGGC2050.015.36461320
GAGGGCC2100.014.9987921
Produced by FastQC (version 0.11.2)