FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_88.35200000039c7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_88.35200000039c7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017076
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20390.2004766605445414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA1453.0286174E-914.48508551
TAGTCCT954.187955E-412.6311244
GACTGTT1456.8960253E-712.4133457
GTATTAT4800.011.8769071
CTATAGT1652.5541704E-711.8177574
ACAGTCC900.00400762111.6662458
CTCTTAG1303.8361773E-511.5403151
TCTATAG1859.1515176E-811.3509423
TTAATAC2701.2732926E-1111.110713
TCCTATA1355.6478195E-511.110712
AGGGCCT950.00599740911.05223410
ACGGTGT2053.200148E-810.9752148
GACCGTC1100.00143286810.9086977
TAGACCT1253.4217458E-410.7996114
CATAGAC1408.174027E-510.71393
ACAGACT1000.00877825710.4996218
ACCGACA1150.002075018110.4344068
TATACAG3650.010.2736025
TAATACA4550.010.2194134
GTGTATG2352.1549567E-810.2144061
Produced by FastQC (version 0.11.2)