FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_87.35200000039c2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_87.35200000039c2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210996
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17850.1473993307987805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTC600.003756036714.9988228
GCGTAAG850.002612320212.3529921
CTAATAC3200.011.7173473
CTGGACC2351.875378E-910.8497644
GCGCACT1000.00877995310.4996111
CCTTGCG2002.6880116E-710.4991769
AATACTA4050.010.3691285
ACCTAAT2651.2460077E-910.1887211
GAGTAGA1501.6430729E-410.0000421
CTATGCA2854.1472958E-109.9992159
ACTATGC2701.724402E-99.9992158
GACCGTC1356.9607235E-49.9992157
TAGACCG1356.963365E-49.9988035
CCTGTAC1501.6451284E-49.9988033
TATACAG3051.364242E-109.8348885
TAGGTAG2003.0450847E-69.7488335
CTATTAA3700.09.729771
ACTGTGC1552.2881795E-49.676668
GGTAGTC1409.71712E-49.64217
AATTATA4050.09.6284762
Produced by FastQC (version 0.11.2)