FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_86.35200000039c01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_86.35200000039c01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089448
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17110.15705201166095123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC400.00651010918.75008813
TAGGACC650.00639139913.8443124
TAGTCAG1454.743015E-813.4464869
TAGGTAG1352.9007242E-713.3315595
TCCGGCC850.002612020812.35299712
TTAGGCT850.002614720312.3512964
ATTATAC3700.011.349843
ACAGGAC1202.3467917E-411.2485023
AGGCCTG950.006002207811.051165
GCGATGG1100.001432523810.90914118
GGTTATA1653.2698426E-610.9091411
CCCGCTT1100.001432523810.90914112
GTAGTCA1807.8819903E-710.8318918
TGGGGTC1253.4252426E-410.7985625
CCCTTAT1951.8937499E-710.769281
TATTAGG3400.010.5868252
GTCCGGC1000.00877629110.50004811
AGTCCGG1000.00878226610.499083510
GGCTATA1150.002074520810.434831
GTATTAG2608.949428E-1010.3846641
Produced by FastQC (version 0.11.2)