FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_85.35200000039bcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_85.35200000039bcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450441
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.19891617326131505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT350.003002122521.430086
TGATTAG400.006501849718.7513230
TAGGACC400.006506019718.7492374
ACTAGTC500.001108275118.0012668
TACCGTT706.023543E-415.0010567
GTCTAGA706.0286216E-414.999391
TAAGACT750.001016570813.999434
CCTATAC650.00638451713.8440533
GGTTAAG800.001654192713.1244651
ATATTAG1601.2045348E-813.1244651
GTACAGG954.180076E-412.6310641
TGTACCA954.180076E-412.6310645
GCATTGG850.002608803612.3524381
GTATAGG900.004001836311.6661911
TATTAGA1355.6382883E-511.1094262
CACTTTG1100.00142891210.9098588
TATTAGG1100.001431563410.9074352
GTGTAAT1253.4139073E-410.799561
AGAGAGT1000.008758604510.5007386
CTATAGT1000.00876581910.4995731
Produced by FastQC (version 0.11.2)