FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_84.35200000039b89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_84.35200000039b89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736307
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8920.12114512017405783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACG400.006508409518.74995812
CGGACGG400.006508409518.74995813
TAGTCAG1102.354136E-816.3635989
TAGGTAG1451.8189894E-1015.5161525
GAGTCCA809.744766E-514.9999669
GCGTCGC851.6322175E-414.11761620
GTATTAG2250.013.3323971
GGTCATA1056.747795E-512.856241
TCTAGAC850.002612097712.3520753
GGTAGTC1354.2052725E-612.2213647
TCAGATG1406.3054104E-611.78568712
CTAGACA900.004006845411.6658484
TTCGTGT1059.703732E-411.42854521
TTAGGTA2301.3078534E-911.0861794
AGTTCAG950.005993237311.05260710
TATCCTG1501.358448E-510.9992285
TCAGTTC1100.001432461410.9083258
TAGACAG1408.169807E-510.7135335
AGACATA1851.1167594E-610.54123229
ATTAGGT1851.1187112E-610.5398013
Produced by FastQC (version 0.11.2)