FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_83.35200000039b46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_83.35200000039b46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856982
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12600.1470275921781321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGGA650.00638816913.84488810
CGATCAC850.002607403612.35541724
GTCTTAG850.00261311712.3518121
GGATAGA1501.0391013E-611.9989031
TCTATAC2301.05501385E-1011.7380573
GGTGCCG900.004008397511.66568
TAGGCAG900.004008397511.665611
GACAGGT1059.713819E-411.4275267
GATTGCG1059.713819E-411.4275267
TAGAACT1602.261011E-611.2489714
CACCGCG950.005995969311.05226723
GTAGACT950.00599857411.0516214
GTATAAC2053.1997843E-810.97460651
ACACTCC1100.001433130610.9080938
CTAGGCA1100.001433130610.90809310
AATACTA4100.010.6087875
ACCTAAT1851.1198263E-610.5395771
GTCTAGG1000.00877997210.499041
TATTATA8600.010.464162
CTAATAC2557.175913E-99.9990863
Produced by FastQC (version 0.11.2)