FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_82.35200000039b1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_82.35200000039b1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173081
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3710.21435050641029343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGT402.4581814E-422.496756
GAGAGAG350.00299847721.4254747
ATAGACA400.00649410318.7472924
CAAGACT400.00649410318.7472924
ATGCAAA400.00649410318.74729211
GTTATAC601.8561987E-417.4974733
AGAGAGA650.006363284313.8441536
TATACCA800.001648323613.1231045
TATTATA1200.00293580449.9985552
ATATACT1500.00180885448.99874
ATATATA2608.13704E-57.4989162
Produced by FastQC (version 0.11.2)