FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_81.35200000039ada.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_81.35200000039ada.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368922
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGCC400.00650994818.7461857
TCACACA500.001106768318.00366230
TAGTTCC500.001109794717.996344
CTTCTAG854.7133108E-717.6410791
CTATGCA601.8642427E-417.496449
ATCACAC550.002095152616.36696629
ACACCGT550.00210085716.360316
ACAGTGC600.0037525614.9969498
TCAGCGG600.0037525614.9969498
TCTAGTA600.003755946914.9949172
TGTAATA1351.8473656E-814.4395492
TAGAAGA1607.9853635E-1014.059644
TAATACT1402.9747753E-813.9257394
TGACCAT650.006383807413.8433385
ACGGTGT650.006383807413.8433388
GTTATAA650.00638954413.8414611
TAATAGA1106.893455E-613.6335914
TATACGG902.648861E-413.3306225
GTATAAA1404.4970147E-712.8527851
ATATTAG1056.747026E-512.8527851
Produced by FastQC (version 0.11.2)