FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_80.35200000039a97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_80.35200000039a97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252548
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5090.20154584475030488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTG200.005142894629.9962375
TTATACT852.201341E-819.409334
TGTACTC559.5508905E-519.0885125
AAGGGTT805.1471034E-616.8728835
GTATTAG550.002097294416.3615821
ATTACCG550.002097294416.3615823
CATTAGA600.003746249514.9981181
CAAGACA600.003746249514.9981184
GTCTTAC706.0194876E-414.9981181
AATACAG600.003746249514.9981185
ATTATAC901.5737298E-514.9981173
CTTGTAC800.001651742313.1233543
AAAACCA954.171761E-412.6299935
TATACTG954.171761E-412.6299935
CTAATAT1006.425074E-411.9984943
TAATATA900.003996039711.6652024
ATACAGT1059.675148E-411.4271376
TATTAAG1355.625413E-511.1075172
TATTATG950.005989084511.0490572
TATTATA1653.250234E-610.9055622
Produced by FastQC (version 0.11.2)