FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_79.35200000039a54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_79.35200000039a54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699801
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12300.17576425298049014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACAGC350.003005282521.4282156
TAGGTAG1007.2523108E-917.99975
GTGCTCC550.002100772516.3633639
GCCGCGT809.73893E-515.00082225
GTATTAG1950.014.6140981
TAGTCAG1054.2647443E-614.2854769
TCGCCGC851.6312444E-414.11842123
CTCCTAA851.6332259E-414.1164031
GTCTAGT650.00638648413.84493351
CTATAAG1106.9064354E-613.6351621
GAGGACT902.6499978E-413.3331124
TACTCTG902.6499978E-413.3331125
AGTACTG902.6499978E-413.3331125
TAATACT3400.012.7939034
AGGTTGC954.1843718E-412.6313696
ACTGGGC954.1843718E-412.6313698
AGGTGCC1354.201913E-612.2220187
GGTAGTC1006.444318E-411.99987
ACTGATC1151.5769537E-411.73893458
CAGTGTT1151.5769537E-411.73893459
Produced by FastQC (version 0.11.2)