FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_78.35200000039a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_78.35200000039a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309032
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41710.18063846668214212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG2950.013.2186315
GCCGCGT850.002609805712.35535825
GTCTTAG2503.6379788E-1211.9989691
TAAGACT4050.011.850324
GGTAGTC3400.011.4691067
CCTTATA3950.011.3909332
CGTGTCG950.00600451311.05144310
GTATTAG6050.010.90815451
TATTAGG5050.010.6916882
TTATACT6400.010.5455124
CACGAGG1000.008768856510.50205425
CTGTGCG1000.0087910.4986439
CTAATAC5650.010.3526453
GTATTAA7050.09.9991411
AGGCCGC1356.9676794E-49.99892410
GAGTGCC1356.969066E-49.9987087
TAATACT9800.09.9476944
TCTATAC5950.09.8306633
GTATTAC6300.09.7610661
GAGTCCG1552.2913769E-49.6761699
Produced by FastQC (version 0.11.2)