FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l04n02_ntr_77.352000000399db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l04n02_ntr_77.352000000399db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604206
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCG954.1919976E-412.63051610
GTATTAG4100.012.4391411
CCTAGCG1101.0417854E-412.27475630
ACTATGC3450.011.3026918
CTTACAC2402.6593625E-910.6234433
GACTGTT2002.6928865E-710.4984637
GTACTAG2156.449227E-810.4652141
GACGATC1150.002076434410.4342322
GGACTGT1304.92324E-410.3830946
TAGGACC1602.7802267E-510.3109884
TATACTG6150.010.2424025
TTATACT7300.010.06701854
CTGTGCA1501.6452947E-49.9991599
TACGGTG2854.1472958E-109.9985357
TAGGACA1653.9009654E-59.9985354
GTATAGA4800.09.687591
TGGACTG1250.0041467779.5985945
CGGTGTT3203.4015102E-109.374219
AGACTGC1450.00134064029.3089816
TAGACTG1450.00134064029.3089815
Produced by FastQC (version 0.11.2)